Amplification of Mycobacterium tuberculosis from peripheral blood

Diagnosis of Tuberculosis by PCR-based Amplification of mpt64 Gene from Peripheral Blood

Shin-Hwar Wu, Cheng-Mao Ho and Jang-Jih Lu Division of Critical Care Medicine, Department of Medicine, Changhua Christian Hospital, Changhua,Taiwan, ROC Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, ROC Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan, ROC Department of Medical Research, China Medical University...

Direct amplification of Mycobacterium tuberculosis deoxyribonucleic acid in paucibacillary tuberculosis.

A STUDY OF MYCOBACTERIUM TUBERCULOSIS DRUG RESISTANCE IN PULMONARY TUBERCULOSIS

Tuberculosis remains a major public health problem in both developing and developed countries. Drug-resistant tuberculosis is an increasing health problem and serious challenge to tuberculosis (TB) control programs. Information about the susceptibility pattern of Mycobacterium tuberculosis isolates against anti-tuberculosis drugs is an important aspect to TB control. The objectives of the s...

Rapid Detection of Mycobacterium tuberculosis by Recombinase Polymerase Amplification

Improved access to effective tests for diagnosing tuberculosis (TB) has been designated a public health priority by the World Health Organisation. In high burden TB countries nucleic acid based TB tests have been restricted to centralised laboratories and specialised research settings. Requirements such as a constant electrical supply, air conditioning and skilled, computer literate operators p...

Detection and identification of Mycobacterium tuberculosis by DNA amplification.

The polymerase chain reaction (PCR) was used to identify mycobacterial DNA sequences in uncultured clinical specimens. Two oligonucleotide primers derived from the sequence of a gene that codes for the 65-kilodalton antigen of Mycobacterium tuberculosis amplified DNA from all 11 species of mycobacteria tested. Amplified DNAs of nontuberculosis mycobacteria were found to be approximately 20 to 4...